Plataformes científiques i Serveis
Genòmica Funcional
Introducció
IRB Barcelona’s Functional Genomics Core Facility (FGC) provides state-of-the-art tools for genomic research, including genome wide expression profiling, DNA copy number-, and SNP analysis. FGC offers a complete solution including consultation, RNA characterisation, microarray processing and data analysis. FGC works preferentially using the Affymetrix GeneChip platform.
Expression profiling and DNA copy number analysis is currently performed for more than 20 different organisms. Tools for ChIP-on-chip analysis are available for human, mouse, Drosophila, yeast and Arabidopsis. SNP analysis is performed for humans.
Expression profiling is performed at three different levels:
- Genome wide measurements of one transcript per gene (3’ biased assays using "standard" arrays)
- Measurement of transcriptional activity for individual exons across the entire genome (Exon Arrays)
- Discovery of novel transcripts using Tiling Arrays
Expression profiling can be performed with as few as 100 cells using NuGEN’s Ribo-SPIA chemistry.
DNA copy number analysis for the measurement of amplification and deletion is performed using "gene resolution analysis of copy number variation" (graCNV) and can be performed for all organisms for which expression arrays are available.
ChIP-on-chip analysis for characterisation of transcription factor binding sites, DNA methylation and histone modifications is performed using Tiling Arrays or Promoter Arrays.
SNP analysis can be performed for 10,000 to more than one million polymorphic sites on a single array.
FGC provides help with experimental design and troubleshooting of nucleic acids isolation and can perform all other steps of microarray processing and data analysis for researchers. For further information contact herbert.auer
irbbarcelona.org and silvia.rodriguezirbbarcelona.org or visit the website www.dnaarrays.org
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