Plataformas Científicas y Servicios

Genómica funcional

Introducción

IRB Barcelona’s Functional Genomics Core Facility (FGC) provides state-of-the-art tools for genomic research, including genome wide expression profiling, DNA copy number-, and SNP analysis. FGC offers a complete solution including consultation, RNA characterisation, microarray processing and data analysis. FGC works preferentially using the Affymetrix GeneChip platform.

Expression profiling and DNA copy number analysis is currently performed for more than 20 different organisms. Tools for ChIP-on-chip analysis are available for human, mouse, Drosophila, yeast and Arabidopsis. SNP analysis is performed for humans.

Expression profiling is performed at three different levels:

1. Genome wide measurements of one transcript per gene (3’ biased assays using "standard" arrays)
2. Measurement of transcriptional activity for individual exons across the entire genome (Exon Arrays)
3. Discovery of novel transcripts using Tiling Arrays

Expression profiling can be performed with as few as 100 cells using NuGEN’s Ribo-SPIA chemistry.

DNA copy number analysis for the measurement of amplification and deletion is performed using "gene resolution analysis of copy number variation" (graCNV) and can be performed for all organisms for which expression arrays are available.

ChIP-on-chip analysis for characterisation of transcription factor binding sites, DNA methylation and histone modifications is performed using Tiling Arrays or Promoter Arrays.

SNP analysis can be performed for 10,000 to more than one million polymorphic sites on a single array.

FGC provides help with experimental design and troubleshooting of nucleic acids isolation and can perform all other steps of microarray processing and data analysis for researchers. For further information contact herbert.auerirbbarcelona.org and silvia.rodriguezirbbarcelona.org or visit the website www.dnaarrays.org

Publications

Transcriptional profiling of the megabladder mouse: a unique model of bladder dysmorphogenesis
Singh S, Robinson M, Ismail I, Saha M, Auer H, Kornacker K, Robinson ML, Bates CM and McHugh KM
Dev Dyn, 237(1), 170-186 (2008)

Drosophila HP1c isoform interacts with the zinc-finger proteins WOC and relative-of-WOC (ROW) to regulate gene expression
Font-Burgada J, Rossell D, Auer H and Azorín F
Genes Dev, 22 (21), 3007-3023 (2008)

More info

Para más información, tarifas, formularios y protocolos visitad nuestra página web www.dnaarrays.org

• 2008 Scientific Report - Functional Genomics Core Facility


• 2007 Scientific Report - Functional Genomics Core Facility