Egfr controls Drosophila tracheal tube elongation by intracellular trafficking regulation
Speaker: Ivette Olivares, PhD student, IBMB-CSIC
Organizer: IRB Barcelona
Date: Wednesday, 26th April 2017, 15.00h
Place: Aula Fèlix Serratosa, Parc Científic de Barcelona, Spain
Host: Marco Milán, IRB Barcelona
Development is governed by a few conserved signalling pathways. Among them, the EGFR pathway is used reiteratively for organ and tissue formation, and when dysregulated can lead to cancer and metastasis. Given its relevance, identifying its downstream molecular machinery and understanding how it instructs cellular changes is of capital importance. Here we approach this issue in the respiratory system of Drosophila. We identify a new role for EGFR restricting the elongation of the tracheal Dorsal Trunk. We find that EGFR regulates the apical determinant Crb and the extracellular matrix regulator Serp, two factors previously known to control tube length. EGFR regulates the organisation of endosomes in which Crb and Serp proteins are loaded. Our results are consistent with a role of EGFR in regulating retromer/WASH recycling routes. Futhermore, we provide new insights into Crb trafficking and recycling during organ formation. Our work connects cell signalling, trafficking mechanisms and morphogenesis and suggests that the regulation of cargo trafficking can be a general outcome of EGFR activation.
Cell and Developmental Biology Programme Seminar