Barcelona BioMed Workshop

Engineering genetic mutations in the mouse

21 Feb 2017

An introduction to current techniques and the best approaches for generating a range of mutations in the mouse. Places reserved for IRB Barcelona alumni.

 

Date: Tuesday, 21st February 2017
Time: 09:30  – 13:00
Place: Fèlix Serratosa,  Parc Cientific de Barcelona (PCB)

 

A range of reverse genetics approaches will be discussed, including DNA insertions by pro-nuclear injection,  and the use of mouse embryonic stem (ES) cells for making targeted mutations

The talk will discuss the use of state of the art sequence specific nucleases, and other techniques currently in development.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The workshop is offered exclusively to IRB members and limited to 60 participants. To register, please send an email to xenia.villalobos@irbbarcelona.org  by 17 February.

09.30 – 09.40 – Introduction IRB Mouse Mutant Facility.

09.40 - 11.10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.

  • DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.

11.10 – 11.30 –   COFFEE BREAK
 
11.30 – 13.00 –The use of sequence specific nucleases for generating mutations, and other methods currenly in development.

  • Crispr´s, TALEN’s, etc.

Stephen Forrow. Mouse Mutant Core facility manager