Pericentriolar satellites: a support network for centrosomes?
Speaker: Dr. Fanni Gergely, Cancer Research UK Cambridge Institute, University of Cambridge, UK
Organizers: IRB Barcelona
Date: Friday 24 February 12:00h
Place: Sala Fèlix Serratosa, Parc Científic de Barcelona, Spain
Host: Dr.Cayetano González, IRB Barcelona
Centrosomes are small cytoplasmic organelles that generate and organise microtubules, and thus contribute to a multitude of cellular processes including cell division and intracellular trafficking. Centrosomes also act as a template for the assembly of the primary cilium, a sensory and signaling organelle. Our group is interested in the role of centrosomes in human pathologies including cancer. Our recent survey of centrosome abnormalities in high-grade serous ovarian cancer revealed that the majority of tumours exhibit severe centrosome aberrancies of both numerical and structural types. The cause of these phenotypes remains obscure, not least because our incomplete understanding of centrosome assembly. Pericentriolar satellites (PS), electron dense cytoplasmic granules that surround the centrosomes, have recently emerged as potential regulators of centrosome function. Moreover, several proteins that localize to PS are mutated in human ciliopathies and neurodevelopmental disorders. Nonetheless, the precise contribution of PS to the various molecular processes and diseases is unclear, in part due to the limited knowledge of their composition. To fill this gap, we performed a systematic mass-spectrometry-based approach and obtained a PS proteome. In addition to presenting functional data on new PS components, I will discuss what we can learn from this proteomic analysis about the cellular function(s) of PS and how we can relate our findings to the centrosome abnormalities seen in ovarian cancer.