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Top-Down Mass Spectrometry Meeting

21 Sep 2015 - 22 Sep 2015

 

 

Top-down mass spectrometry (MS) has emerged as a powerful proteomic approach. Providing unique information on proteins at the molecular level, this technique allows the study of the combinatorial effects of multiple PTMs and/or mutations occurring on the same polypeptide chain (proteoforms) and their physiological function. Moreover, technical advances in Native top-down strategies allow the study of protein complexes (complexoforms). In parallel, top-down approaches performed at a large scale (top-down proteomics) are expected to become viable alternatives to those of bottom-up in terms of protein identification.
However, in contrast to the latter approach, top-down is considerably more challenging because of the complexity of the data generated and various technical limitations and instrumentation requirements.

Participants will be informed about the current state of top-down MS, the types of instrumentation available, and the applications and potentiality of the technique. Leading experts working in top-down MS and top-down proteomics have been invited to explain the tools they use and their latest achievements. The overall objective of the meeting is to speed up the use of top-down MS in proteomic laboratories and to maximize the results by improving the experimental procedures and data analysis.

 

 

There is no registration fee for this meeting, but the number of participants is limited.

Contacts:

marta.vilaseca@irbbarcelona.org

goretti.mallorqui@irbbarcelona.org

The nearest and most convenient hotel is NH Barcelona Stadium Hotel (nearby IRB Barcelona; http://www.nh-hoteles.es/hotel/nh-barcelona-stadium). If you are interested in booking a room, please give the reference for the Top-Down meeting (Reference number 2547001).

Contact: Lourdes Parellada (+34 93 3399050 or L.Parellada@nh-hotels.com).

Day 1, 21st September

12.30-13.45 Registration-lunch

  • 13:45-13:55 Welcome address
  • 13:55-14:15 Introduction to the meeting. Marta Vilaseca, IRB Barcelona, Spain
  • 14:15-15:15 Neil Kelleher.  BMBCB and Feinberg School of Medicine, Northwestern University, Chicago IL, US. “Targeted and Untargeted Analysis of Protein Interactions by Native and Top-down MS”
  • 15:15-16:00 Jim Stephenson.  Thermo Fisher Scientific, Cambridge, MA. “The Evolution of Top-Down Mass Spectrometry and its Application to Microbial Proteomics”

16:00-16:30 Coffee BREAK

  • 16:30-17:15 Frank Sobott. University of Antwerp , Antwerp, Belgium. "Native ion mobility-mass spectrometry: from flexible proteins to ion channels”
  • 17:15-17:45 Martin De Cecco. Waters, Wilmslow, UK. “Improved Workflows for Protein Characterization by Top/Middle-down Fragmentation”
  • 17:45-18:15 Seema Sharma. ThermoFisher, San Jose, CA, US. "Top Down Protein Analysis using Orbitrap Fusion Lumos Tribrid Mass Spectrometer"

20:00 Visit- Moritz Factoy

20:45 WG3 COST Participants Networking dinner

 

Day 2, 22nd September

9.00-9.30 Welcome coffee

  • 9:30-10:15 Yury Tsybin.  Spectroswiss, EPFL Innovation Park, Lausanne, Switzerland. “Advances in Top-down Mass Spectrometry for Antibody and Clinically-Relevant Protein Analysis”
  • 10:15-11:00 Jeremy J. Wolff.  Bruker Daltonics, Billerica, MA, US. “ECD, ETD, and MALDI-ISD Analysis of Large, Intact Biomolecules”

11:00-11:30 Coffee break

  • 11:30-12:15 Julia Chamot-Rooke. Institute Pasteur, Paris, France. "Investigating Bacterial Pathogenicity with Top-Down Proteomics"
  • 12:15-13:00 Helen Cooper.University of Birmingham, Edgbaston, Birmingham, UK. “What’s at the Surface? Latest Developments in LESA Mass Spectrometry of Biological Substrates”

13:00-14:30 Lunch

  • 14:30-15:15 Andrey Dyachenko. Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht, The Netherlands. "High resolution tandem mass spectrometry of intact proteins and protein complexes: from new instrumentation to new applications "
  • 15:15-15:45 Francesc Márquez.  Bruker, Barcelona, Spain. “Rapid generation of accurate information on proteoform distribution and relative abundance by UHR-QTOF MS; an application example”
  • 15:45-16:15 Seema Sharma. Thermo Fisher Scientific, San Jose, CA, US. "ProSight turorial"

16:15-16:45 Concluding remarks. COST-WG3 final discussion- Coffee

The nearest and most convenient hotel is NH Barcelona Stadium Hotel (nearby IRB Barcelona; http://www.nh-hoteles.es/hotel/nh-barcelona-stadium). If you are interested in booking a room, please give the reference for the Top-Down meeting (Reference number 2547001).

Contact: Lourdes Parellada (+34 93 3399050 or L.Parellada@nh-hotels.com).

 

  • Neil Kelleher. BMBCB and Feinberg School of Medicine, Northwestern University, Chicago IL, UL
  • Yury Tsybin. Spectroswiss, EPFL Innovation Park, Lausanne, Switzerland
  • Julia Chamot-Rooke. Institute Pasteur, Paris, France
  • Helen Cooper. University of Birmingham, Edgbaston, Birmingham, UK
  • Frank Sobott. University of Antwerp , Antwerp, Belgium
  • Andrey Dyachenko. University of Utrecht, The Netherlands
  • Jeremy J. Wolff. Bruker Daltonics, Billerica, MA, US
  • Seema Sharma. ThermoFisher, San Jose, CA, US
  • Martin De Cecco. Waters, Manchester, UK
  • Jim Stephenson. BRIMS Center. ThermoFisher. Boston, MA, US
  • Francesc Márquez.  Bruker, Barcelona, Spain

Marta Vilaseca. IRB Barcelona, Barcelona, Spain
COST Action BM 1403-WG3
 

 

 

 

    

To register, please send an email to goretti.mallorqui@irbbarcelona.org by 14th August 2015.

Deadline Extended to 24th August!