Plataforma Científica de Microscopia Digital Avançada
Julien Colombelli
Manager
Microscopia Digital Avançada
Tel Oficina : +34 93 402 04 51
correu-e : julien.colombelli
irbbarcelona.org
Introducció
La Plataforma Científica de Microscopia Digital Avançada ofereix una extensa gama de serveis de microscopia de llum als grups de recerca de l'IRB Barcelona i del PCB. La Plataforma té com a objectiu facilitar l’accés a microscopis d’última generació convencionals i làser, així com oferir suport en tècniques d’imatge avançades, com per exemple FRAP o FRET, per facilitar el processament d’imatges i la seva posterior interpretació i presentació de resultats.
La Plataforma també preveu desenvolupar i combinar tècniques de microscopia per accelerar l’accés a noves aproximacions i promoure noves col·laboracions científiques en el camp de la imatge.
En col·laboració amb el Parc Científic de Barcelona (PCB), la Plataforma de Microscopia Digital Avançada de l'IRB Barcelona/PCB ofereix en l'actualitat accés a microscopis confocals, invertits i verticals convencionals.
Durant el període 2008-2009, la Plataforma donarà accés a d’altres tècniques avançades, incloses la microscopia 2-fotó, el disc giratori confocal, la reflexió total interna (TIRF), la dissecció làser i nanocirugia in vivo, i la imatge automatitzada.
Per a més informació, contacti amb el manager de la Plataforma Científica a
julien.colombelliirbbarcelona.org.
Més informació
Plataforma Científica de Microscopia Digital Avançada
Services for IRB Barcelona and PCB researchers
- Conventional transmission and fluorescence microscopy. Bright field, phase contrast, differential interference contrast (DIC), dark field and multiple colour fluorescence imaging of fixed samples. Fluorescence stereoscopy for sample manipulation and select on, extended focus imaging.
The facility has six units: Inverted epifluorescence (Nikon TE200), upright epifluorescence (Nikon E600, E800, E1000) and fluorescence stereoscopy (Leica), fluorescence Macroscopy (Olympus MVX10).
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Spectral confocal microscopy. 3D, 4D and 5D imaging with optical sectioning, custom spectral detection and resolution, multiple position and incubated environment control for living cells.
The facility has three units: Inverted confocal (Leica SP5, Leica SP2) and upright confocal (SPE).
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Multiphoton microscopy. Deep fluorescence imaging and multiphoton applications. Tailored on the SP5 with an ultrashort pulsed IR laser.
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Spinning disk confocal microscopy. Fast 5D imaging with the Andor Revolution system, equipped with EMCCD camera technology.
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Automated fluorescence Widefield Microscopy with High Content Screening and TIRF.
The facility has one unit: Olympus CellR/ScanR. Fully automated inverted microscope for imaging wide areas, multiwell plates and multipositions. Automated live imaging with temperature incubation and CO2 control. Superstable fluorescence illumination for reproducible and high content image analysis.
Total internal reflection microscopy: High contrast imaging at surfaces.
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Laser manipulation of living cells and organisms. FRAP and laser nanosurgery combined on a wide field fluorescence microscope to perform FRAP and fluorescence patterning, cell ablation, subcellular ablation and correlative microscopy. Laser extraction of prepared tissue sections, single cell or tissue area.
The facility has two units: Zeiss Axiovert 200M, a custom platform developed in collaboration with the European Molecular Biology Laboratory (EMBL),
and an Olympus MMI Microdissector.
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Imaging workstations: Image processing and analysis on 32- and 64-bit workstations equipped with Imaris (Bitplane), Leica LAS AF, Igor Pro, and custom ImageJ tailored solutions.
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Demo System. The A1R spectral confocal microscope is installed in our laboratory, in collaboration with Izasa S.A. Please contact us if you are interested in finding out more and in testing the system.
Upcoming applications in 2010:
Microinjection, Lightsheet Microscopy (SPIM)
Map Legend: 1: SP5 confocal + multiphoton, 2: SP2 confocal, 3: SPE confocal, 4: Epifluorescence microscopes, 5: Fluorescent stereoscope, 6: fluorescence Macroscope, 7: Confocal spinning disk, 8: Microdissector, 9: Laser Nanosurgery + FRAP, 10: Automated widefield for Screening + TIRF, 11: Image Processing workstations.
Plataforma Científica de Microscopia Digital Avançada
Access
Located in Room PBC12, our microscopy systems are available 24 hours a day, 7 days a week, to all scientists working in the Barcelona Science Park.
External scientists and companies can also access our facilities. Please send an official request to microscopyirbbarcelona.org, describing the goal and principles of the experiments.
Billing policies
We apply a 30% discount out of office hours (8am to 9am, 6pm to 10pm) and 50% discount for overnight imaging (from 10pm to 8am on confocal systems).
Booking
During office hours (9am-6pm), access to systems is restricted to a maximum of 4 hours per user. This restriction is released if booking is made less than 48h before the experiment.
The High Content automated microscopy and Long-term Live imaging system (ScanR/CellR) has no time booking restrictions.
Access to our booking application must be granted by the ADM staff for each new user and for each system, with the exception of fluorescence stereoscopy (Lupa), which is accessible to all.
Booking is possible up to 14 days in advance.
Late cancellation (< 24h in advance) will result in billing if the time slot is not reserved by another user.
Some systems might have further booking restrictions (example: number of hours available per group per week). If you experience any problems or need special booking conditions for a specific experiment, please contact us.
To access the instrument booking application form, please click here.
Please note that you will need to accept an SSL certificate when accessing the booking application.
Plataforma Científica de Microscopia Digital Avançada
Manager
Julien Colombelli
tel +34 93 402 04 51
e-mail : julien.colombelliirbbarcelona.org
Research Officers
Lídia Bardia
tel +34 93 403 46 47
e-mail: lidia.bardiairbbarcelona.org
Anna Lladó
tel +34 93 403 46 47
e-mail: anna.lladoirbbarcelona.org
Senior Research Officer
Sébastien Tosi
tel: +34 93 403 46 47
e-mail: sebastien.tosiirbbarcelona.org
Plataforma Científica de Microscopia Digital Avançada
A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation
Timinszky G, Till S, Hassa PO, Hothorn M, Kustatscher G, Nijmeijer B, Colombelli J, Altmeyer M, Stelzer EH, Scheffzek K, Hottiger MO, Ladurner AG.
Nat Struct Mol Biol, 16 (9), 923-929 (2009)
Pulsed forces timed by a ratchet-like mechanism drive directed tissue movement during dorsal closure
Solon J, Kaya-Copur A, Colombelli J, Brunner D.
Cell, 137 (7), 1331-1342 (2009)
Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization
Colombelli J, Besser A, Kress H, Reynaud EG, Girard P, Caussinus E, Haselmann U, Small JV, Schwarz US, Stelzer EH.
J Cell Sci, 122 (Pt 10), 1665-1679 (2009)
A correlative light and electron microscopy method based on laser micropatterning and etching
Colombelli J, Tängemo C, Haselman U, Antony C, Stelzer EHK, Pepperkok R, Reynaud EG.
Methods Mol Biol., 457 (3), 13 (2008)
Tip-cell migration controls stalk-cell intercalation during Drosophila tracheal tube elongation
Caussinus E, Colombelli J, Affolter M.
Curr Biol., 18 (22), 1727-1734 (2008)
Mechanism of phototaxis in marine zooplankton
Jékely G, Colombelli J, Hausen H, Guy K, Stelzer E, Nédélec F, Arendt D.
Nature, 456 (7220), 395-399 (2008)
The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis
Maier P, Rathfelder N, Maeder CI, Colombelli J, Stelzer EH, Knop M.
EMBO J., 27 (18), 2363-2374 (2008)
Investigating relaxation processes in cells and developing organisms: from cell ablation to cytoskeleton nanosurgery
Colombelli J, Reynaud EG, Stelzer EH.
Methods Cell Biol., 82, 267-291 (2007)
Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM)
Engelbrecht CJ, Greger K, Reynaud EG, Krzic U, Colombelli J, Stelzer EH.
Opt Express, 15 (10), 6420-6430 (2007)
Dynein-mediated pulling forces drive rapid mitotic spindle elongation in Ustilago maydis
Fink G, Schuchardt I, Colombelli J, Stelzer E, Steinberg G.
EMBO J, 25 (20), 4897-4907 (2006)
Laser nanosurgery in cell biology
Colombelli J, Pepperkok R, Stelzer EH, Reynaud EG.
Med Sci (Paris), 22 (6-7), 651-658 (2006)
Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
Taxis C, Keller P, Kavagiou Z, Jensen LJ, Colombelli J, Bork P, Stelzer EH, Knop M.
J Cell Biol., 171 (4), 627-640 (2005)
In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery
Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH.
Traffic, 6 (12), 1093-1102 (2005)
Subcellular nanorsurgery with a pulsed subnanosecond UV-A laser
Colombelli J, Reynaud EG, Stelzer EHK.
Med Laser Appl., 20 (2005)
Ultraviolet diffraction limited nanosuregery of live biological tissues
Colombelli, J, Grill, S, Stelzer EHK.
Rev. Sci. Instr., 75 (2), 472-478 (2004)
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