Cell division laboratory
Cayetano González
Group Leader
ICREA Research Professor
Office Tel : +34 93 40 37191
Lab Tel : +34 93 403 70 18
e-mail : gonzalez
irbbarcelona.org
Research Interests
We study the mechanisms of cell division through a multidisciplinary approach that combines genetics, and molecular biology and advanced microscopy. As model systems, we use Drosophila and also cultured cells derived from vertebrates.
Research Lines
Protein Traps: Subcellular localisation is a key aspect of protein function.
We have now automated a protein trap protocol (Reviewed in Gonzalez and Bejarano, 2000) which is being applied in several high-content screens.
Spindle assembly in vivo: Direct visualisation is now mandatory to unravel the complex processes that occur within the living cell. Using protocols developed in our laboratory, we are obtaining new data on the behaviour of specific proteins labelled with florescent tags (Rebollo and Gonzalez , 2000; Lange et al., 2002; Sampaio et al., 2001; Rebollo et al., 2004).
Molecular analysis of centrosomes. (Lange et al., 2002; Tavosanis and Gonzalez, 2003). We have recently cloned the gene that encodes a centriolar protein. Our laboratory has access to mutant alleles, functional GFP fusions and antibodies, that will be all instrumental tools in the molecular dissection of this organelle.
Modelling cancer in Drosophila: We are starting to use Drosophila to study some basic principles regarding cell proliferation and malignant growth (Caussinus and González, 2005; Wodarz & Gonzalez, 2006. Previews, Cell).
Funding
This group is financially supported by the following:
- VI Framework Programme Grants, EU
- Ministerio de Educación y Ciencia (Spanish Ministry of Science and Education)
- Generalitat de Catalunya (Government of Catalonia)
More info
Cell division laboratory
The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts.
Januschke J, Gonzalez C.
J Cell Biol., 188 (5), 693-706 (2010)
Time-lapse imaging of embryonic neural stem cell division in Drosophila by two-photon microscopy.
Rebollo E, Gonzalez C.
Curr Protoc Stem Cell Biol., Chapter 1 (Unit1H.2) (2010)
Structural properties of g,t-parallel duplexes.
Aviñó A, Cubero E, Gargallo R, González C, Orozco M, Eritja R.
J Nucleic Acids. (2010)
Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila
Janic A, Mendizabal L, Llamazares S, Rossell D, Gonzalez C.
Science, 330 (6012) (2010)
Neural stem cells: the need for a proper orientation.
Lesage B, Gutierrez I, Martí E, Gonzalez C.
Curr Opin Genet Dev., 20 (4), 438-42 (2010)
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility.
Watts JK, Martín-Pintado N, Gómez-Pinto I, Schwartzentruber J, Portella G, Orozco M, González C, Damha MJ.
Nucleic Acids Res, 8 (7), 2498-511 (2010)
Spindle alignment is achieved without rotation after the first cell cycle in Drosophila embryonic neuroblasts
Rebollo E, Roldán M and Gonzalez C
Development, 136 (20), 3393-3397 (2009)
Polyhomeotic has a tumor suppressor activity mediated by repression of Notch signaling
Martinez AM, Schuettengruber B, Sakr S, Janic A, Gonzalez C and Cavalli G
Nat Genet, 41 (10), 1076-1082 (2009)
Biased segregation of DNA and centrosomes: moving together or drifting apart?
Tajbakhsh S and Gonzalez C
Nat Rev Mol Cell Biol, 10 (11), 804-810 (2009)
Below the convergence
Gonzalez C
Curr Biol, 19 (8), R313-314 (2009)
Centrosome dysfunction in Drosophila neural stem cells causes tumors that are not due to genome instability
Castellanos E, Dominguez P and González C
Curr Biol, 18 (16), 1209-1214 (2008)
Centrosome function during stem cell division: the devil is in the details
Gonzalez C.
Curr Opin Cell Biol, 20 (6), 694-698 (2008)
Drosophila asymmetric division, polarity and cancer
Januschke J and Gonzalez C
Oncogene, 27 (55), 6994-6702 (2008)
Asterless is a centriolar protein required for centrosome function and embryo development in Drosophila
Varmark H, Llamazares S, Rebollo E, Lange B, Reina J, Schwarz H and González C
Curr Biol, 17 (20), 1735-1745 (2007)
Spindle orientation, asymmetric division and tumour suppression in Drosophila stem cells
González C
Nat Rev Genet, 8 (6), 462-472 (2007)
Functionally unequal centrosomes drive spindle orientation in asymmetrically dividing Drosophila neural stem cells
Rebollo E, Sampaio P, Januschke J, Llamazares S, Varmark H and González C
Dev Cell, 12 (3), 467-474 (2007)
Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes
Dyer N, Rebollo E, Domínguez P, Elkhatib N, Chavrier P, Daviet L, González C and González-Gaitán M
Development, 134 (24), 4437-4447 (2007)
Localized transfection with magnetic beads coated with PCR products and other nucleic acids
Santori MI, Gonzalez C, Serrano L and Isalan M
Nat Protoc, 1 (2), 526-531 (2006)
Connecting cancer to the asymmetric division of stem cells
Wodarz A and Gonzalez C
Cell, 124 (6), 1121-1123 (2006)
Induction of tumor growth by altered stem-cell asymmetric division in Drosophila melanogaster
Caussinus E and Gonzalez C
Nat Genet, 37 (10), 1125-1129 (2005)
Structure and microtubule-nucleation activity of isolated Drosophila embryo centrosomes characterized by whole mount scanning and transmission electron microscopy
Lange BM, Kirfel G, Gestmann I, Herzog V and González C
Histochem Cell Biol, 124 (3-4), 325-334 (2005)
Localized transfection on arrays of magnetic beads coated with PCR products
Isalan M, Santori MI, Gonzalez C and Serrano L
Nat Methods, 2 (2), 113-118 (2005)
Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes
Rebollo E, Llamazares S, Reina J and Gonzalez C
PLoS Biol, 2 (1), 54-64 (2004)
Time-lapse imaging of male meiosis by phase-contrast and fluorescence microscopy
Rebollo E and González C
In “Drosophila Cytogenetics Protocols”, Methods Mol Biol, 247, 77-87 (2004)
gamma-Tubulin function during female germ-cell development and oogenesis in Drosophila
Tavosanis G and Gonzalez C
Proc Natl Acad Sci U SA, 100 (18), 10263-10268 (2003)
Cell division: the place and time of cytokinesis
Gonzalez C
Curr Biol, 13 (9), 363-365 (2003)
Drosophila dd4 mutants reveal that gammaTuRC is required to maintain juxtaposed half spindles in spermatocytes
Barbosa V, Gatt M, Rebollo E, Gonzalez C and Glover DM
J Cell Sci, 116 (Pt 5), 929-941 (2003)
Cell division laboratory
© Photos by Gianluca Battista/Massimiliano Minocri
Cayetano González
Group Leader
ICREA Research Professor
Office Tel : +34 93 40 37191
Lab Tel : +34 93 403 70 18
e-mail : gonzalezirbbarcelona.org
Postdoctoral Fellows
Fabrizio Rossi
tel +34 93 40 37018
e-mail: fabrizio.rossiirbbarcelona.org
Giulia Pollarolo
e-mail: giulia.pollaroloirbbarcelona.org
Jens Januschke
tel +34 93 40 37018
e-mail: januschkeirbbarcelona.org
José Reina
tel +34 93 40 37018
e-mail: reinairbbarcelona.org
Judit Pampalona
tel +34 93 40 37018
e-mail: judit.pampalonairbbarcelona.org
Kazuya Hashiyama
tel +34 93 40 37018
e-mail: kazuya.hashiyamairbbarcelona.org
Zhanna Shcheprova
tel +34 93 40 37018
e-mail: zhanna.shcheprovairbbarcelona.org
PhD Students
Eulàlia Scheenaard
tel +34 93 40 37018
e-mail: eulalia.scheenaardirbbarcelona.org
Research Assistants
Jan Peter Heinen
tel +34 93 40 37018
e-mail: peter.heinenirbbarcelona.org
Salud Llamazares
tel +34 93 40 37018
e-mail: llamazaresirbbarcelona.org
Lab Technicians
Andrea Durán
tel +34 93 40 37018
e-mail: andrea.duranirbbarcelona.org
Administrative Assistant
Sandra Ricol
tel +34 93 40 39982
e-mail: sandra.ricolirbbarcelona.org
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