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GCP8 negatively regulates the γTuRC, inhibiting ciliogenesis and promoting G1/S progression

4 Jul 18

Speaker: Artur Ezquerra, PhD student IRB Barcelona



Organizers: IRB Barcelona

Date: Wednesday, 4 July, 15:00h

Place: Aula Fèlix Serratosa, Parc Científic de Barcelona

Host: Students and postdocs of the C&DB programme.



Primary cilia are microtubule-based structures that organize critical signaling events during development and tissue homeostasis. Ciliogenesis typically takes place at the basal body during G0/G1 phase, when the centrosome migrates to the cell surface to form the basal body. The centrosome contains two centrioles (mother and daughter), being the mother characterized by two projection structures called appendages. While distal appendages are required for cilia formation by docking the centriole to the plasma membrane, sub-distal appendages appear to be dispensable.

GCP8/MZT2 was identified as a subunit of the g-tubulin ring complex (gTuRC), but its function remains largely unknown. We have previously shown that GCP8 is not required for gTuRC assembly/stability and has no obvious role in mitotic spindle assembly, but contributes to gTuRC recruitment and microtubule nucleation at interphase centrosomes.

Unexpectedly, we have recently found that the depletion of GCP8 stimulates cilia formation and promotes cell cycle exit in RPE-1 cells. Here we present our recent advances in deciphering the role of GCP8 during ciliogenesis.


Cell and Developmental Biology Programme Seminar