Barcelona BioMed Workshop

Engineering genetic mutations in the mouse

26 Mar 2020

The first session will concentrate on the well-established methods of classical transgenesis and gene-targeting via embryonic stem cells, and will include examples of how to produce a range of mutation types; for example over-expression models, gene knock-outs and point mutations, both constitutive and conditional.

The second session will address more recent developments, such as the use of gene-editing nucleases, their advantages and limitations, and novel methods of gene transfer.

Date: Thursday, 26 March 2020

Time: 09:30  – 13:00

Place: Fèlix Serratosa,  Parc Cientific de Barcelona (PCB)

The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to leyre.caracuel@irbbarcelona.org by 23 March. 

09:30 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.

DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.

11:10 – 11:30 –   COFFEE BREAK
 
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.

Stephen Forrow. Mouse Mutant Core facility Manager