The first session will concentrate on the well-established methods of classical transgenesis and gene-targeting via embryonic stem cells, and will include examples of how to produce a range of mutation types; for example over-expression models, gene knock-outs and point mutations, both constitutive and conditional.
The second session will address more recent developments, such as the use of gene-editing nucleases, their advantages and limitations, and novel methods of gene transfer.
Date: Thursday, 26 March 2020
Time: 09:30 – 13:00
Place: Fèlix Serratosa, Parc Cientific de Barcelona (PCB)
The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to firstname.lastname@example.org by 23 March.
09:30 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.
DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.
11:10 – 11:30 – COFFEE BREAK
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.
Stephen Forrow. Mouse Mutant Core facility Manager