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Engineering genetic mutations in the mouse

26 Mar 20

The first session will concentrate on the well-established methods of classical transgenesis and gene-targeting via embryonic stem cells, and will include examples of how to produce a range of mutation types; for example over-expression models, gene knock-outs and point mutations, both constitutive and conditional.

The second session will address more recent developments, such as the use of gene-editing nucleases, their advantages and limitations, and novel methods of gene transfer.



Date: Thursday, 26 March 2020

Time: 09:30  – 13:00

Place: Fèlix Serratosa,  Parc Cientific de Barcelona (PCB)

The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to by 23 March. 


09:30 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.

DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.

11:10 – 11:30 –   COFFEE BREAK
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.


Stephen Forrow. Mouse Mutant Core facility Manager