Speaker: Dr. Rafael J. Argüello
CNRS Researcher / ISAC Marylou Ingram Scholar 2021-2023 / “Metabolic Regulation of Immunity” Centre d`Immunologie de Marseille-Luminy, France
Hosts: Alejo Rodríguez-Fraticelli / Manuel Palacín
Node: Cell Pathophysiology / Preclinical Models of Disease
Date: Wednesday 21 September 2022, 12.00h
IMPORTANT: For attendees outside the PCB community you must register at least 24h before the seminar.
Metabolic reprogramming is a hallmark of cancer and immune responses. Different immune cells can adopt pro or anti-tumoral functions that are correlated with the dependence of different energetic metabolism pathways. Metabolic reprogramming involves dramatic changes in cellular levels of NAD+, Lactate, Acetyl-CoA, SAM and other metabolites that are substrates of the enzymes that mediate epigenetic modifications. During immune activation epigenetic changes of the chromatin occur concomitantly to changes in the metabolism. These links between metabolic changes in cancer and immune cells, their impact on epigenetics, gene expression and cell function are poorly understood. Especially due to the lack of adapted technologies that allow to study metabolism and epigenetics with single cell resolution in physiologically relevant contexts.
In this seminar I will present our recent development and applications of a method to monitor functional metabolism with single cell resolution, and our first results using this technology, called SCENITH in combination with measurements of epigenetic marks of the chromatin (Epic-SCENITH). Altogether, SCENITH enable to characterize the metabolic and epigenetic landscapes of cancer and immune cells in patient samples. We envision to use SCENITH as a personalized medicine approach and use its information to predict the efficacy of immunotherapies.