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Engineering genetic mutations in the mouse

29 Ene 19

This session will cover an introduction to current techniques and the best approaches to perform a range of mutations in the mouse, including pro-nuclear injection and the use of mouse embryonic stem cells for gene targeting.

The second part of the session will cover the use of DNA sequence specific nucleases; The current state of the technology and it’s likely future development. 

Places reserved for IRB Barcelona alumni.



Date: Tuesday, 29 January 2019


Time: 09:30  – 13:00

Place: Fèlix Serratosa,  Parc Cientific de Barcelona (PCB)

The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to by 25 January. 


09:30 – 09:40 – Introduction to the IRB Mouse Mutant Facility.

09:40 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.

  • DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.

11:10 – 11:30 –   COFFEE BREAK
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.


Stephen Forrow. Mouse Mutant Core facility Manager