Barcelona BioMed Workshop

Engineering genetic mutations in the mouse

29 Jan 2019

This session will cover an introduction to current techniques and the best approaches to perform a range of mutations in the mouse, including pro-nuclear injection and the use of mouse embryonic stem cells for gene targeting.

The second part of the session will cover the use of DNA sequence specific nucleases; The current state of the technology and it’s likely future development. 

Places reserved for IRB Barcelona alumni.

Date: Tuesday, 29 January 2019

 

Time: 09:30  – 13:00

Place: Fèlix Serratosa,  Parc Cientific de Barcelona (PCB)

The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to leyre.caracuel@irbbarcelona.org by 25 January. 

09:30 – 09:40 – Introduction to the IRB Mouse Mutant Facility.

09:40 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.

  • DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.

11:10 – 11:30 –   COFFEE BREAK
 
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.

Stephen Forrow. Mouse Mutant Core facility Manager