This session will cover an introduction to current techniques and the best approaches to perform a range of mutations in the mouse, including pro-nuclear injection and the use of mouse embryonic stem cells for gene targeting.
The second part of the session will cover the use of DNA sequence specific nucleases; The current state of the technology and it’s likely future development.
Places reserved for IRB Barcelona alumni.
Date: Tuesday, 29 January 2019
Time: 09:30 – 13:00
Place: Fèlix Serratosa, Parc Cientific de Barcelona (PCB)
The workshop is offered exclusively to IRB and BIST members and limited to 40 participants. To register, please send an email to firstname.lastname@example.org by 25 January.
09:30 – 09:40 – Introduction to the IRB Mouse Mutant Facility.
09:40 - 11:10 – An introduction into techniques for generating genetic mutations in the mouse germ-line.
- DNA insertions by pro-nuclear injection, use of mouse embryonic stem cells, gene targeting and other methods.
11:10 – 11:30 – COFFEE BREAK
11:30 – 13:00 –The application of DNA sequence specific nucleases in generating mutations; current and future use.
Stephen Forrow. Mouse Mutant Core facility Manager