Top-down protein mass spectrometry allows comprehensive characterization of proteoforms from complex mixtures and avoids many pitfalls associated with traditional bottom-up workflows. Moreover, proteoform analysis is strongly connected to diagnosis in complex phenotypes and can be key in clinical applications.
We will present several examples of top-down mass spectrometry for its use in translational proteomics and the latest advances in the field with Orbitrap instruments.
9:20 Marta Vilaseca (IRB Barcelona): Welcome and Introduction.
9:30 Marina Gay (IRB Barcelona): Proteoforms. The new proteomics challenge
10:15 Luca Fornelli (University of Oklahoma): Applications of proton transfer charge reduction for intact protein characterization.
10:55 Daniel Lopez-Ferrer (Thermo Fisher, San José, CA): New technological advances for Top-down protein analysis on an Orbitrap Eclipse tribrid MS.
11:35 Coffee break
12:05 Peter Verhaert (ProteoFormiX, Belgium): Development of different clinical Top-down proteomics applications
12:45 Sega Ndiaye (Thermo Fisher, Paris, France): Advances in Orbitrap Instrumentation for Native Top-Down Analysis of Non- Covalent Protein Complexes.
13:15 Daniel Lopez-Ferrer (Thermo Fisher, San José, CA): NanoPOTS sample processing and the Orbitrap Eclipse tribrid mass spectrometer enable in-depth proteome profiling of single mammalian cells
13:45 Meeting ends
13:50 Lunch and networking