Serveis i Equipaments Principals Histopathology Facility

Neus Prats

Core Facility Manager

+34 93 40 20546

The facility provides histopathological services and training opportunities to the IRB scientific community and to external public and private research centers, thus applying its expertise to offer customized and cost-effective support.

The facility offers a wide variety of histological studies, ranging from routine techniques and services to the development of new approaches (IHC, IF,…), experimental pathology studies, reports, and consultancy services (mouse phenotyping, animal models of disease, drug efficacy and non-GLP toxicity studies).

Facility staff are also involved in the organization and provision of specialized courses and master’s degree in Laboratory Animal Pathology and Toxicology.

For more information, please contact the facility manager at

neus.prats@irbbarcelona.org

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Services

Rodent histopathological studies and reports:
- Assistance with protocol study set up (experimental design) and development
- Mouse phenotyping
- Animal models of disease (inflammatory, autoimmune, neoplastic)
- Drug efficacy
- Non-GLP drug toxicity
Whole rodent necropsy, tissue collection, gross examination with report.
- Training in necropsy techniques, including blood collection and perfusion
Fixation of cells, tissues and organs
Trimming, freezing and embedding of tissues for histology
Paraffin-embedded and frozen sections
Routine Hematoxylin and Eosin staining of sections
Specialized histochemical staining of sections (e.g. Trichrome, PAS, Pearl´s, and many other stains)
Immunohistochemistry (IHC) and Immunofluorescence (IF) studies
Digital image scanning
Quantitative Techniques for Morphological Evaluation (Histomorphometry, Computer-based Image Analysis)

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Facility equipment

Tissue-Tek VIP Vacuum Infiltration Processor - Sakura
RM2255 Rotary Microtome – Leica
CM1950 Cryostat – Leica
CoverStainer – Dako
Artisan Link Pro – Dako
AutostainerPlus – Dako
Ventana Discovery XT – Roche
CX43 Biological Microscope – Oympus
DM1000 LED bright field microscope with ICC50 HD digital camera – LEICA
Nanozoomer 2.0HT – Hamamatsu

Selected publications

Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging.

Salzer MC, Lafzi A, Berenguer-Llergo A, Youssif C, Castellanos A, Solanas G, Peixoto FO, Stephan-Otto Attolini C, Prats N, Aguilera M, Martín-Caballero J, Heyn H, Benitah SA.

Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging. Cell, 6 (175), doi: 10.1016/j.cell.2018.10.012-1575-1590 (2019)

Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice.

Terré B, Lewis M, Gil-Gómez G, Han Z, Lu H, Aguilera M, Prats N, Roy S, Zhao H, Stracker TH.

Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice. Development, 8 (146), doi: 10.1242/dev.162628 (2019)

BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis.

Welz PS, Zinna VM, Symeonidi A, Koronowski KB, Kinouchi K, Smith JG, Guillén IM, Castellanos A, Furrow S, Aragón F, Crainiciuc G, Prats N, Caballero JM, Hidalgo A, Sassone-Corsi P, Benitah SA.

BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis. Cell, 6 (177), doi: 10.1016/j.cell.2019.05.009-1436-1447 (2019)

Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours.

Avgustinova A, Symeonidi A, Castellanos A, Urdiroz-Urricelqui U, Solé-Boldo L, Martín M, Pérez-Rodríguez I, Prats N, Lehner B, Supek F, Benitah SA.

Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours. Nat Cell Biol., 12 (20), doi: 10.1038/s41556-018-0233-x-1400-1409 (2018)

Targeting metastasis-initiating cells through the fatty acid receptor CD36.

Pascual G, Avgustinova A, Mejetta S, Martín M, Castellanos A, Attolini CS, Berenguer A, Prats N, Toll A, Hueto JA, Bescós C, Di Croce L, Benitah SA.

Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature, 7635 (541), doi: 10.1038/nature20791.-41-45 (2017)

A genome editing approach to study cancer stem cells in human tumors.

Cortina C, Turon G, Stork D, Hernando-Momblona X, Sevillano M, Aguilera M, Tosi S, Merlos-Suárez A, Stephan-Otto Attolini C, Sancho E, Batlle E.

A genome editing approach to study cancer stem cells in human tumors. EMBO Mol Med., 7 (9), doi: 10.15252/emmm.201707550.-869-879 (2017)

Loss of Dnmt3a and Dnmt3b does not affect epidermal homeostasis but promotes squamous transformation through PPAR-γ.

Rinaldi L, Avgustinova A, Martín M, Datta D, Solanas G, Prats N, Benitah SA.

Loss of Dnmt3a and Dnmt3b does not affect epidermal homeostasis but promotes squamous transformation through PPAR-γ. Elife, doi: 10.7554/eLife.21697. (2016)