Serveis i Equipaments Principals Histopathology Facility
The facility provides histopathological services and training opportunities to the IRB scientific community and to external public and private research centers, thus applying its expertise to offer customized and cost-effective support.
The facility offers a wide variety of histological studies, ranging from routine techniques and services to the development of new approaches (IHC, IF,…), experimental pathology studies, reports, and consultancy services (mouse phenotyping, animal models of disease, drug efficacy and non-GLP toxicity studies).
Facility staff are also involved in the organization and provision of specialized courses and master’s degree in Laboratory Animal Pathology and Toxicology.
For more information, please contact the facility manager at
- Rodent histopathological studies and reports:
- Assistance with protocol study set up (experimental design) and development
- Mouse phenotyping
- Animal models of disease (inflammatory, autoimmune, neoplastic)
- Drug efficacy
- Non-GLP drug toxicity
- Whole rodent necropsy, tissue collection, gross examination with report.
- Training in necropsy techniques, including blood collection and perfusion
- Training in necropsy techniques, including blood collection and perfusion
- Fixation of cells, tissues and organs
- Trimming, freezing and embedding of tissues for histology
- Paraffin-embedded and frozen sections
- Routine Hematoxylin and Eosin staining of sections
- Specialized histochemical staining of sections (e.g. Trichrome, PAS, Pearl´s, and many other stains)
- Immunohistochemistry (IHC) and Immunofluorescence (IF) studies
- Digital image scanning
- Quantitative Techniques for Morphological Evaluation (Histomorphometry, Computer-based Image Analysis)
- Tissue-Tek VIP Vacuum Infiltration Processor - Sakura
- RM2255 Rotary Microtome – Leica
- CM1950 Cryostat – Leica
- CoverStainer – Dako
- Artisan Link Pro – Dako
- AutostainerPlus – Dako
- Ventana Discovery XT – Roche
- CX43 Biological Microscope – Oympus
- DM1000 LED bright field microscope with ICC50 HD digital camera – LEICA
- Nanozoomer 2.0HT – Hamamatsu
Salzer MC, Lafzi A, Berenguer-Llergo A, Youssif C, Castellanos A, Solanas G, Peixoto FO, Stephan-Otto Attolini C, Prats N, Aguilera M, Martín-Caballero J, Heyn H, Benitah SA.
Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging. Cell, 6 (175), doi: 10.1016/j.cell.2018.10.012-1575-1590 (2019)
Terré B, Lewis M, Gil-Gómez G, Han Z, Lu H, Aguilera M, Prats N, Roy S, Zhao H, Stracker TH.
Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice. Development, 8 (146), doi: 10.1242/dev.162628 (2019)
Welz PS, Zinna VM, Symeonidi A, Koronowski KB, Kinouchi K, Smith JG, Guillén IM, Castellanos A, Furrow S, Aragón F, Crainiciuc G, Prats N, Caballero JM, Hidalgo A, Sassone-Corsi P, Benitah SA.
BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis. Cell, 6 (177), doi: 10.1016/j.cell.2019.05.009-1436-1447 (2019)
Avgustinova A, Symeonidi A, Castellanos A, Urdiroz-Urricelqui U, Solé-Boldo L, Martín M, Pérez-Rodríguez I, Prats N, Lehner B, Supek F, Benitah SA.
Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours. Nat Cell Biol., 12 (20), doi: 10.1038/s41556-018-0233-x-1400-1409 (2018)
Pascual G, Avgustinova A, Mejetta S, Martín M, Castellanos A, Attolini CS, Berenguer A, Prats N, Toll A, Hueto JA, Bescós C, Di Croce L, Benitah SA.
Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature, 7635 (541), doi: 10.1038/nature20791.-41-45 (2017)
Cortina C, Turon G, Stork D, Hernando-Momblona X, Sevillano M, Aguilera M, Tosi S, Merlos-Suárez A, Stephan-Otto Attolini C, Sancho E, Batlle E.
A genome editing approach to study cancer stem cells in human tumors. EMBO Mol Med., 7 (9), doi: 10.15252/emmm.201707550.-869-879 (2017)
Rinaldi L, Avgustinova A, Martín M, Datta D, Solanas G, Prats N, Benitah SA.
Loss of Dnmt3a and Dnmt3b does not affect epidermal homeostasis but promotes squamous transformation through PPAR-γ. Elife, doi: 10.7554/eLife.21697. (2016)
