Histopathology Facility

The Histopathology Core Facility is a centralized service that provides a full range of histopathological resources and training opportunities to the IRB Barcelona scientific community, as well as to external public and private biomedical centres. Thanks to its highly qualified staff and specialized equipment, the facility is able to offer tailored, cost-effective services and expert support.

For more information, please contact histopathology@irbbarcelona.org

Meet the team

Consult the service rates

Whole rodent necropsy, tissue collection, gross examination with report
Hematology and biochemical chemistry analysis
Training in necropsy techniques, including blood collection and perfusion
Sample fixation (cell pellets, organoids, tissues and organs, bone fixation and decalcification)
Tissue trimming, freezing fresh tissue in OCT and formalin fixed paraffin embedding (FFPE) tissue for histology
Tissue sectioning (FFPE and OCT)
Routine Hematoxylin and Eosin staining of tissue sections
Specialized histochemical staining of sections (e.g. Trichrome, PAS, Pearl´s, and many other stains)
Immunohistochemistry (IHC), Immunofluorescence (IF) and FISH techniques including multiplexing
Tissue slide scanning (bright field and IF)
Histopathological and Image Analysis studies and reports
Assistance with protocol study set up (experimental design) and development
Mouse pathology phenotyping
Characterization of different animal models of disease (inflammatory, autoimmune, cancer, ageing, ...)
Histological studies to assess Drug efficacy and toxicity

Initial inquiry: send an email to histopathology@irbbarcelona.org explaining your project needs.
Review & approval: CF staff reviews the request and, if needed, contacts the user for clarification or to schedule a consultation. A quote is sent to the user/PI with the estimated timeline.
Sample Submission: the user fills the study request form and delivers the sample following facility’s guidelines.
Service processing: the facility performs the requested service.
Data Analysis (if applicable): data is processed, analysed, and interpreted according to the service requested.
Results Delivery: results are delivered to the user as a report, raw data, analysis or interpretation.
Follow-up: optional meeting to explain results or assist with interpretation.

Defective Slc7a7 transport reduces erythropoietin compromising erythropoiesis

Giroud-Gerbetant, Judith; Sotillo, Fernando; Hernández, Gonzalo; Ruano, Irene; Sebastián, David; Fort, Joana; Sánchez, Mayka; Weiss, Günter; Prats, Neus; Zorzano, Antonio; Palacín, Manuel; Bodoy, Susanna

Molecular Medicine,

(1 )

(2025)

The efficacy of chemotherapy is limited by intratumoral senescent cells expressing PD-L2

Chaib S; López-Domínguez JA; Lalinde-Gutiérrez M; Prats N; Marin I; Boix O; García-Garijo A; Meyer K; Muñoz MI; Aguilera M; Mateo L; Stephan-Otto Attolini C; Llanos S; Pérez-Ramos S; Escorihuela M; Al-Shahrour F; Cash TP; Tchkonia T; Kirkland JL; Abad M; Gros A; Arribas J; Serrano M

Nature Cancer,

(3 )

448

- 462

(2024)

Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair

Kovatcheva, M; Melendez, E; Chondronasiou, D; Pietrocola, F; Bernad, R; Caballe, A; Junza, A; Capellades, J; Holguín-Horcajo, A; Prats, N; Durand, S; Rovira, M; Yanes, O; Attolini, CSO; Kroemer, G; Serrano, M

Nature Metabolism,

(11 )

1911

- 1930

(2023)

Targeting lymphoid-derived IL-17 signaling to delay skin aging

Solá, P; Mereu, E; Bonjoch, J; Casado-Peláez, M; Prats, N; Aguilera, M; Reina, O; Blanco, E; Esteller, M; Di Croce, L; Heyn, H; Solanas, G; Benitah, SA

Nature Aging,

(6 )

688

(2023)

Iron accumulation drives fibrosis, senescence and the senescence-associated secretory phenotype

Maus, M; López-Polo, V; Mateo, L; Lafarga, M; Aguilera, M; De Lama, E; Meyer, K; Sola, A; Lopez-Martinez, C; López-Alonso, I; Guasch-Piqueras, M; Hernandez-Gonzalez, F; Chaib, S; Rovira, M; Sanchez, M; Faner, R; Agusti, A; Diéguez-Hurtado, R; Ortega, S; Manonelles, A; Engelhardt, S; Monteiro, F; Attolini, CSO; Prats, N; Albaiceta, G; Cruzado,...

Nature Metabolism,

(5 )

2111

- 2130

(2023)

Local IL-17 orchestrates skin aging

Solá, Paloma; Mereu, Elisabetta; Bonjoch, Júlia; Casado, Marta; Reina, Oscar; Blanco, Enrique; Esteller, Manel; DiCroce, Luciano; Heyn, Holger; Solanas, Guiomar; Benitah, Salvador Aznar

Biorxiv : The Preprint Server For Biology,

(2022)

Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming

Chondronasiou, D; Gill, D; Mosteiro, L; Urdinguio, RG; Berenguer, A; Aguilera, M; Durand, S; Aprahamian, F; Nirmalathasan, N; Abad, M; Martin-Herranz, DE; Otto-Attolini, CS; Prats, N; Kroemer, G; Fraga, MF; Reik, W; Serrano, M

Aging Cell,

(3 )

e13578

(2022)

Dietary palmitic acid promotes a prometastatic memory via Schwann cells

Pascual, G; Dominguez, D; Elosua-Bayes, M; Beckedorff, F; Laudanna, C; Bigas, C; Douillet, D; Greco, C; Symeonidi, A; Hernandez, I; Gil, SR; Prats, N; Bescos, C; Shiekhattar, R; Amit, M; Heyn, H; Shilatifard, A; Benitah, SA

Nature,

599

(7885 )

485

(2021)

Pulmonary glycogen deficiency as a new potential cause of respiratory distress syndrome

Testoni, G; Olmeda, B; Duran, J; López-Rodríguez, E; Aguilera, M; Hernández-Alvarez, MI; Prats, N; Pérez-Gil, J; Guinovart, JJ

Human Molecular Genetics,

(21 )

3554

- 3565

(2020)

BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis

Welz, PS; Zinna, VM; Symeonidi, A; Koronowski, KB; Kinouchi, K; Smith, JG; Guillén, IM; Castellanos, A; Crainiciuc, G; Prats, N; Caballero, JM; Hidalgo, A; Sassone-Corsi, P; Benitah, SA

Cell,

177

(6 )

1436

- 1447

(2019)

Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice

Terré, B; Lewis, M; Gil-Gómez, G; Han, ZY; Lu, H; Aguilera, M; Prats, N; Roy, S; Zhao, HT; Stracker, TH

Development,

146

(8 )

dev162628

(2019)

Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours

Avgustinova, A; Symeonidi, A; Castellanos, A; Urdiroz-Urricelqui, U; Sole-Boldo, L; Martin, M; Perez-Rodriguez, I; Prats, N; Lehner, B; Supek, F; Benitah, SA

Nature Cell Biology,

(12 )

1400

(2018)

Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging

Salzer, MC; Lafzi, A; Berenguer-Llergo, A; Youssif, C; Castellanos, A; Solanas, G; Peixoto, FO; Attolini, CSO; Prats, N; Aguilera, M; Martín-Caballero, J; Heyn, H; Benitah, SA

Cell,

175

(6 )

1575

(2018)

Loss of dnmt3a and dnmt3b does not affect epidermal homeostasis but promotes squamous transformation through PPAR-g

Rinaldi, L; Avgustinova, A; Martín, M; Datta, D; Solanas, G; Prats, N; Benitah, SA

Elife,

e21697

(2017)

A genome editing approach to study cancer stem cells in human tumors

Cortina, C; Turon, G; Stork, D; Hernando-Momblona, X; Sevillano, M; Aguilera, M; Tosi, S; Merlos-Suárez, A; Attolini, CSO; Sancho, E; Batlle, E

Embo Molecular Medicine,

(7 )

869

- 879

(2017)

Targeting metastasis-initiating cells through the fatty acid receptor CD36.

Pascual, G; Avgustinova, A; Mejetta, S; Martín, M; Castellanos, A; Attolini, CSO; Berenguer, A; Prats, N; Toll, A; Hueto, JA; Bescós, C; Di Croce, L; Benitah, SA

Nature,

541

(7635 )

(2017)