Date: Tuesday, 11 June, 2019
Time: 09:30 – 13:00
Place: Fèlix Serratosa, Parc Cientific de Barcelona (PCB)
This short workshop will describe many aspects of heterologous expression in E.coli, from choice of construct design through to methods to improve the levels of soluble expression and options for co-expression. In addition, it will also address expression in eukaryotic hosts, including secreted proteins and ECDs, and options for co-expression.
The workshop will finish with a discussion and problem-solving session using examples brought along by the attendees*.
To register, please send and email to firstname.lastname@example.org by June 5, 2019.
9.30-10.00 Introduction to PECF-Nick Berrow
Genes to proteins: Low or High Through-Put approaches, overview of construct design, fusion proteins and purification tags, different expression hosts and strains.
10.00-11.15 E.coli-the expression ‘work-horse’-Raquel Garcia
Including basics and background theory, protein engineering, cloning and expression methods, purification approaches and co-expression methods for complexes.
11.15 – 11.45 – COFFEE BREAK
11.45 – 12.30 – Eukaryotic hosts -Nick Berrow
Including overviews of current mammalian and baculoviral systems, secreted proteins, membrane proteins, and co-expression methods for complexes.
12.30 – 13.00 – Discussion and problem solving
*If you have a protein-related problem that you would like to troubleshoot within the group please send a one-slide explanation in advance of the course.