The Regulome of regeneration
Speaker: Elena Vizcaya, PhD student. Department of Genetics, Microbiology and Statistics. Institut of Biomedicine (IBUB), University of Barcelona.
Organizer: IRB Barcelona
Date: Wednesday, 21 June 2017, 15.00h
Place: Aula Fèlix Serratosa, Parc Científic de Barcelona, Spain
Host: Marco Milán, IRB Barcelona
A key issue in regenerative biology is to understand the transcriptional program and regulatory elements that respond to tissue damage. Drosophila imaginal discs are epithelia that activate a regenerative response after cell death induction or physical injury. To identity regulatory elements governing tissue regeneration we performed genome-wide analyses (ATAC-Seq and RNA-Seq) at different time points (0, 15 and 25 hours) after cell death induction in the wing imaginal disc. Comparison between control and damaged discs shows more regions of accessible chromatin immediately after cell death, which correlates with higher number of up-regulated genes detected at the same moment. Both ATAC and RNA profiles recover the normal levels at 25h after cell death. A fraction of up-regulated genes tend to be located in nearby genomic regions, inindicating co-regulation of gene expression during regeneration. Individual interactions between accessible regions and clusters of co-regulated have been confirmed by 3C analyses. In addition, selected enhancers have been experimentally validated after different types of damage, such as cell death and physical injury. Moreover, regions of open chromatin can be classified in: 1) specific regions accessible only after cell death; 2) non-specific regions that become more accessible after cell death but are also present in control discs, probably corresponding to developmental regulatory regions. Finally, we found differences in chromatin marks distribution and transcription factor enrichment among the two classes of accessible regions, which suggests these regions may play a specific role during the process of regeneration.
Cell and Developmental Biology Programme Seminar