Massively Parallel Human Systems Biology in Synthetic Organisms
Speaker: Jesse Rinehart, PhD, Associate Professor of Cellular & Molecular Physiology. System Biology Institute. Yale University School of Medicine. CT 06520. USA
Organizers: IRB Barcelona
Date: Friday 11 October, 12.00h
Place: Felix Serratosa Hall, Parc Cientific de Barcelona
Host: Lluís Ribas de Pouplana, PhD - IRB Barcelona
Protein phosphorylation encompasses a central cellular language that determines every facet of normal cellular biology. We have recently created a technology that enables site-specific incorporation of phosphoserine into proteins by expanding the genetic code of Escherichia coli. I will describe our new capability to synthesize and observe phosphoproteome-scale libraries of human phosphoproteins that enable answers to systems level questions regarding the functional role of all human phosphorylation events. Our work has also advanced the protein-protein interaction field by enabling the first system for large-scale genetic screening of phosphorylation dependent protein-protein interactions.