- ch-TOG cooperates with a microtubule initiating complex, thus favouring the formation of new microtubules, when the cell is not dividing.
- The work by the Microtubule Organization in Cell Proliferation and Differentiation laboratory has been published in the journal Nature Communications.
Microtubules are cellular fibres that are part of the skeleton of the cell, termed cytoskeleton. They ensure that cells maintain their shape, carry out their functions, and divide. Microtubule fibres are assembled from units of a protein called tubulin and they grow in length by incorporating additional units of tubulin.
Researchers led by Dr. Jens Lüders at IRB Barcelona have discovered a key role played by the ch-TOG protein in the initiation of microtubule assembly during the interphase stage of the cell cycle—the phase in which cells spend most time. The cell cycle is a series of stages that lead to cell growth and division into two daughter cells. The period between cell formation and cell division is known as the interphase. During this time, the cytoskeleton is essential, playing a very active role in maintaining cell functions.
“The role of the ch-TOG protein in stimulating microtubule growth, in particular during cell division, has been described previously, but its roles in human interphase cells, that is to say, in cells that are not dividing, were not clear until now. Our work reveals that, in addition to helping microtubule ends to elongate, this protein is crucial for initiating formation of these fibres,” explains Dr. Lüders, head of the Microtubule Organization in Cell Proliferation and Differentiation laboratory at IRB Barcelona.
A transitory interaction
The mechanism that the researchers have described in the journal Nature Communications is based on the transient binding of ch-TOG at the centrosome—a major site of microtubule formation in the cell. The interaction of ch-TOG with a microtubule initiating complex, the tubulin ring complex, faciliates the binding of several tubulin molecules, and microtubules begin to form. After the initiation, ch-TOG remains attached to the end of the newly formed microtubule fibre, which continues to grow as new tubulin molecules are incorporated.
The scientific community has long considered that ch-TOG served mainly to elongate microtubules. However, this work supports the theory, as proposed by previous studies in vitro and in other organisms, that human ch-TOG is also crucial for the initiation and formation of new microtubule fibres.
“The ch-TOG protein has two regions, one engages the tubulin molecules and the other binds to the microtubule in such a way that ch-TOG adds tubulin to the fibre, allowing it to grow. We have observed that the same two regions are also required for ch-TOG to bind to tubulin ring complexes at centrosomes and start adding tubulin, thus forming a new fibre from scratch,” explains Dr. Aamir Ali, first author of the article, former postdoctoral researcher at IRB Barcelona, now at the Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, United States
The researchers have also observed that, in human cells, ch-TOG’s role in initiating the formation of microtubule fibres is not only observed at the centrosome. ch-TOG can also stimulate the initiation of microtubule fibres from the surface of the Golgi apparatus, another important site of microtubule assembly in certain cell types.
Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG
Aamir Ali, Chithran Vineethakumari, Cristina Lacasa and Jens Lüders
Nature Communications (2022) DOI: 10.1038/s41467-023-35955-w
About IRB Barcelona
The Institute for Research in Biomedicine (IRB Barcelona) pursues a society free of disease. To this end, it conducts multidisciplinary research of excellence to offer pioneering solutions to unresolved medical needs in cancer and other diseases related to ageing. It establishes technology transfer agreements with the pharmaceutical industry and major hospitals to bring research results closer to society and organises a range of science outreach activities to engage the public in an open dialogue. IRB Barcelona is an international centre that hosts 400 employees and more than 30 nationalities. Recognised as a Severo Ochoa Centre of Excellence since 2011, IRB Barcelona is a CERCA centre and member of the Barcelona Institute of Science and Technology (BIST).