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Mecanismos de las Enfermedades
Development and Growth Control Laboratory
Imagen
Group Leader
Telephone:
+34 93 40 34902

ICREA Research Professor

Imagen
ICREA

Research information

Research interests

During the development of multicellular organisms, body growth is controlled at the scale of the organism by the activity of long-range signaling molecules, mostly hormones. These systemic factors coordinate growth between developing tissues and act as relays to adjust body growth in response to environmental changes. In target organs, long-range signals act in concert with tissue-autonomous ones to regulate the final size of a given tissue.

The interplay between systemic and tissue-autonomous signals contributes to defining the final size of a tissue or organ. Drosophila imaginal discs, simple epithelial invaginations that grow one thousand fold in mass and cell number, are probably one of the best model systems to analyze at the genetic and molecular level the control of size in a growing epithelium. My lab uses the wing imaginal disc to dissect the cellular and molecular mechanisms underlying the regulation of tissue growth.

We take an integrative approach as we aim to understand how the final size of the developing wing is achieved not only during normal development but also in stress conditions. In this regard, we are dissecting the cellular and molecular mechanisms underlying the homeostatic capacity of the tissue to several insults (with a special interest in genomic instability) and its potential impact in tumorigenesis. This integrative approach helps to understand the robust interplay between systemic and tissue-autonomous signals in normal development or in stress situations, and contributes to identifying emerging stress signalling molecules transiently induced to compensate for tissue loss that can contribute to tumorigenesis in a condition of chronic expression.

 

Research lines

  1. Morphogens and tissue growth: Morphogens are signalling molecules expressed in restricted domains that spread to the rest of the tissue to form a concentration gradient. A complex set of interactions between morphogens and their corresponding signalling pathways contributes to organizing limb growth along the dorsal-ventral, anterior-posterior and proximal-distal axes. We are currently analyzing how these morphogens and their gradients regulate wing growth.

  2. Systemic hormones and tissue growth: Wing primordia are embedded in the open circulatory system of feeding larva, and the activity of the major nutrient sensing pathways modulates the final size of the developing wing according to nutrient availability. We are currently analyzing the interplay between nutrient sensing pathways and tissue-autonomous signals, including morphogens and their gradients, in regulating wing growth.

  3. Chromosomal instability and tissue homeostasis. Chromosomal instability (CIN) often results in aneuploidy, which compromises cell fitness and leads to cell death. Despite the dramatic levels of apoptosis observed in wing primordia subject to CIN, the impact on the size of the resulting adult wing is mild. We are currently dissecting the molecular mechanisms used at the cellular and tissue-wide level to dampen the deleterious effects of CIN in the wing epithelium.

  4. Chromosomal instability and tumorigenesis. Wing primordia subjected to CIN are characterised by tumor-like overgrowths, metastatic behavior and malignancy to the host upon blockade of the apoptotic machinery, and this tumorigenic response relies mainly on the production of highly aneuploid cells that delaminate from the epithelium and activate a JNK-dependent transcriptional response. We are currently dissecting the cellular and molecular mechanisms underlying CIN-induced tumor growth, metastatic behavior and malignancy.

 

Selected publications

Joy J, Barrio L, Santos-Tapia C, Romão D, Giakoumakis NN, Clemente-Ruiz M, Milán M.
Developmental Cell
( )
-
(2021)
Romao, D; Muzzopappa, M; Barrio, L; Milan, M
Current Biology
31
(8 )
1780 -
+
(2021)
Barrio L; Milán M
Developmental Cell
52
(5 )
659 -
672.e3
(2020)
Murcia L; Clemente-Ruiz M; Pierre-Elies P; Royou A; Milán M
Cell Reports
28
(1 )
119 -
131.e4
(2019)
Benhra N; Barrio L; Muzzopappa M; Milán M
Developmental Cell
47
(2 )
161 -
174.e4
(2018)
Muzzopappa M; Murcia L; Milán M
Proceedings Of The National Academy Of Sciences Of The United States Of America
114
(35 )
E7291 -
E7300
(2017)
Recasens-Alvarez C; Ferreira A; Milán M
Nature Communications
8
(13815 )
13815 -
(2017)
Clemente-Ruiz, Marta; Murillo-Maldonado, Juan M; Benhra, Najate; Barrio, Lara; Perez, Lidia; Quiroga, Gonzalo; Nebreda, Angel R; Milan, Marco
Developmental Cell
36
(3 )
290 -
302
(2016)
Boulan L; Martín D; Milán M
Current Biology
23
(6 )
473 -
478
(2013)
Dekanty, Andres; Barrio, Lara; Muzzopappa, Mariana; Auer, Herbert; Milan, Marco
Proceedings Of The National Academy Of Sciences Of The United States Of America
109
(50 )
20549 -
20554
(2012)
Mesquita D; Dekanty A; Milán M
Plos Biology
8
(12 )
e1000566 -
(2010)
Herranz H; Hong X; Pérez L; Ferreira A; Olivieri D; Cohen S; Milán M
Embo Journal
29
(10 )
1688 -
1698
(2010)
Becam I; Fiuza UM; Arias AM; Milán M
Current Biology
20
(6 )
554 -
560
(2010)
Herranz H, Pérez L, Martín FA, Milán M
Embo Journal
27
(11 )
1633-1645 -
1645
(2008)

Projects

Grup de Recerca consolidat (SGR 2017-2019) de la Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya. Agencia de Gestió d'Ajuts Universitaris i de Recerca (AGAUR). Referencia: 2017 SGR 443.

AGAUR
Secretaria d'Universitas i Recerca

"Control del cercimiento y señalización celular” (INTEGROWTH), cofinanciado por el Ministerio de Economía, Industria y Competitividad - Agencia Estatal de Investigación y por el Fondo Europeo de Desarrollo Regional (FEDER) de la Unión Europea, una manera de hacer Europa. Referencia: BFU2016-77587-P.

Ministerio de ciencia, innovación y universidades
Agencia estatal de investigación
European union

"Control del crecimiento en desarrollo, homeostasis y tumorigénesis" (HOMEOGROWTH), financiado por la Agencia Estatal de Investigación (PID2019-110082GB-I00 / EAI / 10.13039/501100011033).

Agencia estatal de investigación

Group members

Group Leader
Research Associate
Research Associate
PhD Student
PhD Student
PhD Student
Lab Technician
MSc Student
Visiting Student

Group News

IRB Barcelona en los medios
11 Jul 2019
IRB Barcelona en los medios
21 Dic 2017