Cell and Developmental BiologyDevelopment and Growth Control Laboratory

Development and Growth Control Laboratory
Group Leader

ICREA Research Professor

+34 93 40 34902

During the development of multicellular organisms, body growth is controlled at the scale of the organism by the activity of long-range signaling molecules, mostly hormones. These systemic factors coordinate growth between developing tissues and act as relays to adjust body growth in response to environmental changes. In target organs, long-range signals act in concert with tissue-autonomous ones to regulate the final size of a given tissue.

The interplay between systemic and tissue-autonomous signals contributes to defining the final size of a tissue or organ. Drosophila imaginal discs, simple epithelial invaginations that grow one thousand fold in mass and cell number, are probably one of the best model systems to analyze at the genetic and molecular level the control of size in a growing epithelium. My lab uses the wing imaginal disc to dissect the cellular and molecular mechanisms underlying the regulation of tissue growth.

We take an integrative approach as we aim to understand how the final size of the developing wing is achieved not only during normal development but also in stress conditions. In this regard, we are dissecting the cellular and molecular mechanisms underlying the homeostatic capacity of the tissue to several insults (with a special interest in genomic instability) and its potential impact in tumorigenesis. This integrative approach helps to understand the robust interplay between systemic and tissue-autonomous signals in normal development or in stress situations, and contributes to identifying emerging stress signalling molecules transiently induced to compensate for tissue loss that can contribute to tumorigenesis in a condition of chronic expression.

 

  1. Morphogens and tissue growth: Morphogens are signalling molecules expressed in restricted domains that spread to the rest of the tissue to form a concentration gradient. A complex set of interactions between morphogens and their corresponding signalling pathways contributes to organizing limb growth along the dorsal-ventral, anterior-posterior and proximal-distal axes. We are currently analyzing how these morphogens and their gradients regulate wing growth.
  2. Systemic hormones and tissue growth: Wing primordia are embedded in the open circulatory system of feeding larva, and the activity of the major nutrient sensing pathways modulates the final size of the developing wing according to nutrient availability. We are currently analyzing the interplay between nutrient sensing pathways and tissue-autonomous signals, including morphogens and their gradients, in regulating wing growth.
  3. Chromosomal instability and tissue homeostasis. Chromosomal instability (CIN) often results in aneuploidy, which compromises cell fitness and leads to cell death. Despite the dramatic levels of apoptosis observed in wing primordia subject to CIN, the impact on the size of the resulting adult wing is mild. We are currently dissecting the molecular mechanisms used at the cellular and tissue-wide level to dampen the deleterious effects of CIN in the wing epithelium.
  4. Chromosomal instability and tumorigenesis. Wing primordia subjected to CIN are characterised by tumor-like overgrowths, metastatic behavior and malignancy to the host upon blockade of the apoptotic machinery, and this tumorigenic response relies mainly on the production of highly aneuploid cells that delaminate from the epithelium and activate a JNK-dependent transcriptional response. We are currently dissecting the cellular and molecular mechanisms underlying CIN-induced tumor growth, metastatic behavior and malignancy.

 

Terriente-Félix A, Pérez L, Bray SJ, Nebreda AR and Milán M.
Dis Model Mech, 10 (4), 399-407 (2017)
Recasens-Alvarez C, Ferreira A and Milán M.
Nat Commun, 8 13815 (2017)
Clemente-Ruiz M, Murillo-Maldonado JM, Benhra N, Barrio L, Pérez L, Quiroga G, Nebreda AR and Milán M.
Dev Cell, 36 (3), 290-302 (2016)
Boulan L, Martín D and Milán M.
Curr Biol, 23 (6), 473-8 (2013)
Dekanty A, Barrio L, Muzzopappa M, Auer H, and Milán M
P Natl Acad Sci Usa, 109 (50), 20549-54 (2012)
Duran J, Tevy MF, Garcia-Rocha M, Calbó J, Milán M* and Guinovart JJ*.
EMBO Mol Med, 4 (8), 719-29 (2012)
Mesquita D, Dekanty A and Milán M.
Plos Biol, 8 (12), e1000566 (2010)
Becam I, Fiuza UM, Arias AM and Milán M.
Curr Biol, 20 (6), 554-60 (2010)
Herranz H, Hong X, Pérez L, Ferreira A, Olivieri D, Cohen SM and Milán M.
Embo J, 29 (10), 1688-98 (2010)
Herranz H, Pérez L, Martín FA and Milán M.
Embo J, 27 (11), 1633-45 (2008)

* shared senior authorship

This group receives financial support from the following sources:

  • Ministerio de Educación y Ciencia (Spanish Ministry of Science and Education)
  • Generalitat de Catalunya (DURSI)(Government of Catalonia)
  • ICREA (Catalan Institute of Research and Advanced Studies)
  • EMBO Young Investigator Programme
  • Ministerio de Economía y Competitividad (MINECO)
  • European Commission (EC), Fondo Europeo de Desarrollo Regional (FEDER), "Una manera de hacer Europa"

     

Group news & mentions

<p>IRB Barcelona will host students for a research project on the fruit fly (Drosophila melanogaster).</p>
10 Jul 2017

Today marks the start of the second edition of the Barcelona International Youth Science Challenge (BIYSC), aimed at stimulating scientific talent am

<p>The fruit fly (Drosophila melanogaster), a means to model and study human disease.</p>
23 Mar 2017

IRB Barcelona is to participate in the second edition of the Barcelona International Youth Science Challenge (BIYSC), organised by the

<p>Teachers and scientists working together.</p>
17 Mar 2017

On 17 and 18 March, fifteen secondary school teachers from around Catalonia will have the opportunity to learn about the fruit fly Drosophila melanogaster, an animal model used to study de

Upcoming events

20 Sep
Auditorium, Parc Científic de Barcelona
Speaker:
Speaker: Isabel Almudi, Ph.D. Developmental Biology, Evolutionary Biology Andalusian Center for Developmental Biology, Sevilla, Spain.
13 Nov - 15 Nov
Institut d'Estudis Catalans (IEC), Barcelona
Speaker:
Modesto Orozco, Marc Martí-Renom, Giacomo Cavalli
15 Nov - 17 Nov
Place: Palauet Casades, carrer de Mallorca 283, Barcelona (Spain)