The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona. We are able to offer a comprehensive service, for the generation of genetically altered mice including:

  • Design of recombinant DNA molecules for genetic modification.
  • Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
  • All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
  • Microinjection of DNA or ES cells into pre-implantation stage embryos.
  • Transfer of manipulated embryos into pseudo-pregnant foster mice.
  • Subsequent processing of pups born to pseudopregnant fosters.

The facility has a fully equipped laboratory for cell and molecular biology. Mouse embryonic stem (ES) cell culture is carried out within a dedicated cell culture laboratory.

We also have a small microinjection suite based within the PCB Animal Research Center (SEA). This suite contains inverted microscopes equipped with state of the art micromanipulators and microinjectors. The suite also contains everything necessary for mouse surgery and embryo manipulation.

We are equipped to carry out cryopreservation of mouse germ-line tissue.

Access to the facility is by consultation. Please contact us for more information at
mousemutantirbbarcelona.org

Ros S, Zafra D, Valles-Ortega J, García-Rocha M, Forrow S, Domínguez J, Calbó J and Guinovart JJ.
J Biol Chem, 285 (48), 37170-7 (2010)

People

Group news & mentions

4 Dec 2015

Today La Vanguardia publishes a feature article about the CRISPR/Cas9 technique.

29 Dec 2014

“El Periódico de Catalunya” has published an article about a new technique, called Crispr/Cas9, to modify the genome.

Upcoming events

20 Sep
Aula Fèlix Serratosa, Parc Científic de Barcelona
Speaker:
Speaker: Isabel Almudi, Ph.D. Developmental Biology, Evolutionary Biology Andalusian Center for Developmental Biology, Sevilla, Spain.
22 Sep
Serratosa Room, Parc Científic de Barcelona
Speaker:
Speaker: Maurizio Scaltriti, PhD Memorial Sloan Kettering Cancer Center New York, USA
27 Sep
Aula Fèlix Serratosa, Parc Científic de Barcelona
Speaker:
Speaker: Cristina Pujades, Ph.D. Group Leader of the Department of Experimental and Health Sciences, University Pompeu Fabra, PRBB, Barcelona, Spain.