The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona. We are able to offer a comprehensive service, for the generation of genetically altered mice including:

• Design of recombinant DNA molecules for genetic modification.
• Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
• All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
• Microinjection of DNA or ES cells into pre-implantation stage embryos.
• Transfer of manipulated embryos into pseudo-pregnant foster mice.
• Subsequent processing of pups born to pseudopregnant fosters.