Mouse Mutant
The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona for use in the study of development and disease. The facility has been working since January 2007 and is staffed by scientists with extensive experience in cell culture, embryo manipulation and molecular biology.
The facility is able to provide a comprehensive service ranging from initial design of genetic modifications and the vectors required to generate these modifications, through vector construction, and subsequent embryo manipulation to introduce the mutations.
GM mice are generated using a range of techniques and technologies, including mouse embryonic stem cell (mESC) modification, Crispr/Cas9, embryo microinjection, embryo electroporation and embryo transfer.
Access to the facility is by consultation. Please contact us for more information at mousemutant@irbbarcelona.org
We are able to offer a comprehensive service, for the generation of genetically altered mice including:
- Design of recombinant DNA molecules for genetic modification.
- Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
- All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
- Microinjection of DNA or ES cells into pre-implantation stage embryos.
- Transfer of manipulated embryos into pseudo-pregnant foster mice.
Others: 2x Nikon Eclipse TE200, Self-contained tissue culture room
The facility has a fully equipped laboratory for cell and molecular biology. Mouse embryonic stem (ES) cell culture is carried out within a dedicated cell culture laboratory.
We also have a small microinjection suite based within the PCB Animal Research Center (SEA). This suite contains inverted microscopes equipped with state of the art micromanipulators and microinjectors. The suite also contains everything necessary for mouse surgery and embryo manipulation.
We are equipped to carry out cryopreservation of mouse germ-line tissue.
- Request submission: contact mousemutant@irbbarcelona.org to discuss your project.
- Review & approval once the project is discussed and approved, a quote is sent to the user/PI.
- Reagent design/generation/purchasing. CF staff design and synthesise or purchase as required, all vectors and other reagents required for the project.
- Generation of mice/cells, as required.
- Genotyping of GM modified mice/cells
- Transfer of genetically modified mice/cell to end user. A report will be delivered, detailing the generation of the mice, genotyping assays, sequence files etc.
Access to the facility is by consultation. Please contact us for more information at
mousemutant@irbbarcelona.org
