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Mouse Mutant

The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona for use in the study of development and disease. The facility has been working since January 2007 and is staffed by scientists with extensive experience in cell culture, embryo manipulation and molecular biology.

The facility is able to provide a comprehensive service ranging from initial design of genetic modifications and the vectors required to generate these modifications, through vector construction, and subsequent embryo manipulation to introduce the mutations.

GM mice are generated using a range of techniques and technologies, including mouse embryonic stem cell (mESC) modification, Crispr/Cas9, embryo microinjection, embryo electroporation and embryo transfer.

Access to the facility is by consultation. Please contact us for more information at mousemutant@irbbarcelona.org

Consult the service rates

We are able to offer a comprehensive service, for the generation of genetically altered mice including:

  • Design of recombinant DNA molecules for genetic modification.
  • Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
  • All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
  • Microinjection of DNA or ES cells into pre-implantation stage embryos.
  • Transfer of manipulated embryos into pseudo-pregnant foster mice.
  • Request submission: contact mousemutant@irbbarcelona.org to discuss your project.
  • Review & approval once the project is discussed and approved, a quote is sent to the user/PI.
  • Reagent design/generation/purchasing.  CF staff design and synthesise or purchase as required, all vectors and other reagents required for the project.
  • Generation of mice/cells, as required.
  • Genotyping of GM modified mice/cells
  • Transfer of genetically modified mice/cell to end user. A report will be delivered, detailing the generation of the mice, genotyping assays, sequence files etc.
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Access to the facility is by consultation. Please contact us for more information at
mousemutant@irbbarcelona.org

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