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Mechanisms of Disease
Cell Signaling
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Group Leader
Telephone:
+34 93 40 37110
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Affiliated Group Leader
Telephone:
+34 93 40 39895

Research information

Research interests

We aim to unravel how cells detect and respond to environmental changes. We focus our studies on the characterisation of stress signal transduction pathways, especially those regulated by MAP kinases of the Hog1/p38 family, also known as the stress-activated MAP kinases (SAPKs). Proper adaptation to stress involves the modulation of several basic aspects of cell biology, among them the cell cycle and gene expression. Using S. cerevisiae budding yeast as a model organism, as well as higher eukaryotic cells, we are dissecting the molecular mechanisms underlying cell response to changes in the extracellular environment and characterising the adaptive responses required for cell survival. Based on our knowledge of signal transduction and using synthetic biology, we also seek to modify cell behaviour to reprogram cell response to specific inputs/stimuli.

Research lines

1. SAPK signalling: Using quantitative data in single cells and mathematical modelling, together with mutational analyses, we study the basic signalling properties of stress-responsive MAP pathways and how to alter them.

2. SAPK targets: Using proteomics, biochemistry and genetics, our main goal is to identify new targets for SAPKs and thus widen our understanding of cellular adaptation to stress. This information is expected to facilitate the characterisation of the bases of adaptation in eukaryotes.

3. Cell cycle control: SAPKs act in several phases of the cell cycle to allow prompt response to extracellular stimuli and the maintenance of cell integrity. We are uncovering the mechanisms by which Hog1 and p38 SAPKs regulate the cell cycle.

4. Regulation of mRNA biogenesis: SAPKs control critical steps of mRNA biogenesis and are thus key regulators of stress-responsive gene expression. Our main aim is to determine the contribution of multiple factors to overall gene expression in response to stress. We are also using genome-wide CRISPR screening to identify essential genes for stress adaptation.

5. Synthetic biology: We are interested in implementing complex engineered networks to perform in vivo cellular computation. In a joint effort between theoretical and experimental groups, we have established biological circuits with distributed computation. We are now addressing the application of cellular computation to relevant health issues, such as diabetes.

Selected publications

Viéitez C; Martínez-Cebrián G; Solé C; Böttcher R; Potel CM; Savitski MM; Onnebo S; Fabregat M; Shilatifard A; Posas F; de Nadal E
Nucleic Acids Research
48
(7 )
3455 -
3475
(2020)
Tognetti S; Jiménez J; Viganò M; Duch A; Queralt E; de Nadal E; Posas F
Proceedings Of The National Academy Of Sciences Of The United States Of America
117
(16 )
8924 -
8933
(2020)
Amat R; Böttcher R; Le Dily F; Vidal E; Quilez J; Cuartero Y; Beato M; De Nadal E; Posas F
Genome Research
29
(1 )
18 -
28
(2019)
Duch A; Canal B; Barroso S; García-Rubio M; Seisenbacher G; Aguilera A; De Nadal E; Posas F
Nature Communications
9
(1 )
379 -
(2018)
Gubern A; Joaquin M; Marquès M; Maseres P; Garcia-Garcia J; Amat R; González-Nuñez D; Oliva B; Real F; de Nadal E; Posas F
Molecular Cell
64
(1 )
25 -
36
(2016)
Nadal-Ribelles M; Mas G; Millán-Zambrano G; Solé C; Ammerer G; Chávez S; Posas F; De Nadal E
Nucleic Acids Research
43
(10 )
4937 -
4949
(2015)
Nadal-Ribelles M; Solé C; Xu Z; Steinmetz LM; deNadal E; Posas F
Molecular Cell
53
(4 )
549 -
561
(2014)
Duch, Alba; Felipe-Abrio, Irene; Barroso, Sonia; Yaakov, Gilad; Garcia-Rubio, Maria; Aguilera, Andres; de Nadal, Eulalia; Posas, Francesc
Nature
493
(7430 )
116 -
119
(2013)
Joaquin M; Gubern A; González-Nuñez D; Josué Ruiz E; Ferreiro I; De Nadal E; Nebreda A; Posas F
Embo Journal
31
(13 )
2952 -
2964
(2012)
De Nadal E; Ammerer G; Posas F
Nature Reviews Genetics
12
(12 )
833 -
845
(2011)
Solé C; Nadal-Ribelles M; Kraft C; Peter M; Posas F; De Nadal E
Embo Journal
30
(16 )
3274 -
3284
(2011)
Regot S; MacIa J; Conde N; Furukawa K; Kjellén J; Peeters T; Hohmann S; De Nadal E; Posas F; Solé R
Nature
469
(7329 )
207 -
211
(2011)
Escote, X; Zapater, M; Clotet, J; Posas, F
Nature Cell Biology
6
(10 )
997 -
+
(2004)
De Nadal E; Zapater M; Alepuz P; Sumoy L; Mas G; Posas F
Nature
427
(6972 )
370 -
374
(2004)

Projects

"Regulación transcripcional mediante modificaciones de histonas" Ministerio de Ciencia, Innovación y Universidades and Fondo Europeo de Desarrollo Regional (FEDER-UE). Reference: BFU2017-85152-P.

Ministerio de ciencia, innovación y universidades
Agencia estatal de investigación
European union

“Regulation of retinoblastoma (Rb) as a new therapeutic strategy for the treatment of breast cancer” Asociación Española contra el Cancer (AECC). Reference: PROYE18010POSA

AECC

"Circuits cel·lulars sintètics encapsulats per restablir control glicèmic en diabetis mellitus tipus 1" Fundació La Marató de TV3. Reference 201610.31

Fundació La Marató

Grup de Recerca consolidat (SGR 2017-2019) Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya. Agencia de Gestió d'Ajuts Universitaris i de Recerca (AGAUR). Reference: 2017 SGR 779

AGAUR
Secretaria d'Universitas i Recerca

“Disección de la Adaptación al Estrés en Eucariotas” Ministerio de Ciencia, Innovación y Universidades and Fondo Europeo de Desarrollo Regional (FEDER). Reference: PGC2018-094136-B-100

Ministerio de ciencia, innovación y universidades
Agencia estatal de investigación
European union

Group members

Group Leader
Affiliated Group Leader
Research Assistant
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
PhD Student
PhD Student
PhD Student
PhD Student
PhD Student
PhD Student
Lab Technician
MSc Student
MSc Student
Visiting Student